In the realm of advanced biochemical research, the precision 3X FLAG Peptide, lyophilized p | F4799-25MG of protein pur 3X FLAG® Peptide, 1 X 4 mg (F4799-4MG) ification and detection is paramount. My personal experience with high-quality reagents has shown that the 3x flag peptide f4799 stands out as a critical tool for those w High-purity 3X FLAG Peptidefor efficient protein tagging and detection in biochemical research, ensuring reliable performance and quality from APExBIO. orking with fusion proteins. Whether you are performing competitive elution or verifying protein expression, understanding the technical specifications of this synthetic peptide of 23 amino acid residue is essential.
Technical Specifications and Composition
The 3x flag peptide (specifically Product No. F 4799) is a highly engineered tricosapeptide designed for specific applications in immunoaffinity chromatography. With a molecular weight of approximately 2864 Da, this peptide contains the Asp-Tyr-Lys-Xaa-Xaa-Asp motif repeated three times, which grants it superior binding affinity compared to the standard single FLAG-tag.
Key technical attributes include:
* Sequence: MDYKDHDGDYKDHDIDYKDDDDK
* Length: 23 amino acids
* Purity: Typically >95% for consistent experimental results
* Storage: Recommended at 2–8 °C to maintain structural integrity
Practical Applications in the Lab
When I utilize 3x flag peptide in my own experimental setups, the primary advantage is the ability to achieve 3x flag peptide can be used for protein separation and purification under native conditions. Unlike harsh elution methods that may denature sensitive proteins, competitive elution using this 3x flag pep 3X Flag-tag Peptide -20571ES tide allows for the gentle release of fusion proteins from affinity resins such as AntiFLAGBeads.
The 3X FLAG® Peptide is particularly effective because it allows researchers to detect fusion proteins up to 10 fmol, providing the sensitivity required for low-abundance targets. It is important to note that a standard by C Set-Up—3X FLAG® Peptide. F4799. Storage Temperature 2-8 °C. Product Description. The 3X FLAG® Peptide is a synthetic peptide of. 23 amino acid residues, with a Read more FLAG peptide will not effectively elute 3x flag fusion proteins; therefore, utilizing the correct F4799 catalog item is necessary for success.
Why Quality Matters
When sourcing High-purity 3X FLAG Peptide, I prioritize consistency. Whether you are purchasing the 3X FLAG® Peptide, 1 X 4 mg (F4799-4MG) or larger quantities, the Merck 3X FLAG Peptide (or Sigma-Aldrich equivalent) remains the industry benchmark. This 3X concentrated tag is a peptide sequence that is formed by the combination of 23 amino acids, and its hydrophilic nature makes it highly soluble in aqueous buffers.
Integrating the 3x Flag Peptide into Your Workflow
For those investigating 3X DYKDDDDK Tag Peptide functionality, the synthetic peptide of 23 amino acid residues offers a robust solution for:
1. Competitive Elution: Safely removing proteins from 3x flag affinity columns.
2. Protein Detection: Serving as a reliable standard in Western blotting or ELISA-like formats.
3. Research Versatility: Providing a 3x flag peptide solution that works across various expression systems.
In summary, the 3x flag peptide f4 Merck 3X FLAG Peptide; 소모품. F4799-4MG. 재고문의재고: -. Merck F4799-4MG 3X FLAG Peptide, 4 MG pk. CAS: -단위: pk. 754,600원. (VAT포함)830,060원. 문의Read more 799 is an indispensable asset for any laboratory focusing on high-resolution protein analysis. By ensuring you are using a high-quality synthetic peptide of 23 amino acid residue, you can maximize the yield and purity of your 3x flag-tagged proteins while maintaining their native conformation. Always ensure you are checking the 3x flag peptide f4799 price and availability through authorized suppliers to guarantee the authenticity of your reagents.