In the realm of biochemical research and protein purification, the 3xFLAG peptide has become an indispensable tool for scientists. As someone who has spent significant time in the lab working with protein expression and affinity chromatography, I have found that understanding the precise 3xflag peptide size and structural characteristics is essential for successful experimental outcomes.
Defining the Entity: What is the 3xFLAG Peptide?
The 3xFLAG peptide is a synthetic, hydrophilic 23-mer peptide designed to provide high-affinity binding for purification applications. Unlike the original single FLAG tag, the 3xFLAG version incorporates 3X FLAG® Peptide - Sigma-Aldrich three repeated Asp-Tyr-Lys-Xaa-Xaa-Asp motifs. This increased length significantly enhances the binding affinity of the 3x FLAG tag to anti-FLAG antibodies, making it a superior choice for competitive elution.
When discussing the molecular weight of this construct, it is generally cited as approximately 2,861.87 Da (or roughly 2.9 kDa). Its small size is one of its most advantageous features. Because it is a compact peptide, it minimizes interference with the tertiar 3X FLAG peptide y structure and biological function of the target protein, which is critical during sensitive procedures like protein crystallization.
Practical Considerations: Size and Usage
When sourcing this reagent, you will encounter various product formats. Whether you are looking for 10 mg quantities for large-scale purification or smaller aliquots like Size: 1.0 mg or Size, 4mg, 25mg, the quality of the peptides remains consistent. For those ma MDYKDHDGDYKDHDIDYKDDDDK peptide ; Price, 0.00 ; Cat. No. RP21087-10 ; Size.10 mg. Buy in bulk ; Synonyms, DYKDDDDK tag;Flag-tag;3xFlag ; Description, A fusion Read more naging bulk inventory, you might encounter options like 3x FLAG peptid 3X FLAG Peptide for Elution e (25 mg; 5 x 5 mg), which allows for better stability by keeping unused portions stored at -20°C.
The 3xFLAG peptide (often referred to in literature as 3X FLAG peptide or simply 3xFlag) is typically provided as a lyophilized powder. When reconstituting, I have found that following the specific supplier’s protocol—often involving a buffer like TBS—ensures the highest recovery rate. The 3X FLAG peptides are highly specific for competitive elution, meaning they can effectively displace tagged proteins from affinity resins such as the ANTI-FLAG M2 gel.
Key Technical Specifications
* Sequence: The 3X FLAG peptide sequence is characterized by the MDYKDHDGDYKDHDIDYKDDDDK motif.
* Molecular Weight: Approximately 2,862 g/mol.
* Purity: Typically >95% or >96% as determined by HPLC.
* Application: Ideal for SDS-PAGE, immunoprecipitation (IP), and protein identification.
Why the 3xFLAG Peptide Matters
In my own research, the shift from sing Size: 1.0 mg; Physical State: Lyophilized ; Applications: SDS-PAGE, IP ; Description: Triple FLAG® Peptide - 000-000-H47 ; Synonyms: Read more le to triple flags was a game-changer. The 3X FLAG peptide provides a much cleaner elution profile. Because of its small size, it does not easily aggregate, and its hydrophilic nature ensures that it remains soluble during the purification process. Whether you are working with 3X FLAG peptide (TFA) formulations or standard salt forms, the consistency of these peptides allows for highly reproducible results in western blotting and downstream protein analysis.
Final Thoughts on Selection
Choosing the right Size for your 3X Flag requirements depends on your throughput. For routine lab work, I usually keep a few vials of 3xFLAG peptide on hand. Always check the certificate of analysis for the molecular weight and ensure the storage conditions are strictly maintained at -20°C t This 3×Flag repeated polypeptide contains 23 amino acids and can detect fusion proteins up to 10 fmol. 3×Flag repeatedpeptideshave been widely used to purify Read more o preserve the integrity of the 3xFLAG construct. By respecting these technical parameters, you can ensure that your protein purification workflows remain ef 3X FLAG peptide (TFA) - MedChem Express ficient and reliable.