In the realm of biochemical r 3X FLAG peptidesare FLAG-taggedpeptidescontaining three repeats of the Asp-Tyr-Lys-Xaa-Xaa-Asp motif.3X FLAG peptidecan be used for protein separation Read more esearch and protein purification, the 3xFLAG peptide has become an indispensable tool for scientists. As someone who has spent significant time in the lab working with protein expression and affinity chromatography, I have found that understanding the precise 3xflag peptide size and structural characteristics is essential for successful experimental outcomes.
Defining the Entity: What is the 3xFLAG Peptide?
The 3xFLAG peptide is a synthetic, hydrophilic 23-mer peptide designed to provide high-affinity binding for purification applications. Unlike the original single FLAG tag, the 3xFLAG version incorporates three repeated Asp-Tyr-Lys-Xaa-Xaa-Asp motifs. This increased length significantly enhances the binding affinity of the 3x FLAG tag to anti-FLAG antibodies, making it a superior choice for competitive elution.
When discussing the molecular weight of this construct, it is generally cited as approximately 2,861.87 Da (or roughly 2.9 kDa). Its small siz This 3×Flag repeated polypeptide contains 23 amino acids and can detect fusion proteins up to 10 fmol. 3×Flag repeatedpeptideshave been widely used to purify Read more e is one of its most advantageous features. Because it is a compact peptide, it minimizes interference with the tertiary structure and biological function of the target protein, which is critical during sensitive procedures like protein crystallization.
Practical Considerations: Size and Usage
When sourcing this reagent, you will encounter various product formats. Whether you are looking for 10 mg quantities for large-scale purification or smaller aliquots like Size: 1.0 mg or Size, 4mg, 25mg, the quality of the peptides remains consistent. For those managing bulk inventory, you might encounter options like 3x FLAG peptide (25 mg; 5 x 5 mg), which allows for better stability by keeping unused portions stored at -20°C.
The 3xFLAG peptide (often referred to in literature as 3X FLAG peptide or simply 3xFlag) is typically provided as a lyophilized powder. When reconstituting, I have found that following the specific supplier’s protocol—often involving a buffer like TBS—ensures the highest re 3X FLAG peptide | Protein Marker covery rate. The 3X FLAG peptides are highly specific for competitive elution, meaning they can effectively displace tagged proteins from affinity resins such as the ANTI-FLAG M2 gel.
Key Technical Specifications
* Sequence: The 3X FLAG peptide sequence is characterized by the MDYKDHDGDYKDHDIDYKDDDDK motif.
* Molecular Weight: Approximately 2,862 g/mol.
* Purity: Typically >95% or >96% as determined by HPLC.
* Ap 3X FLAG peptide | Protein Marker plication: Ideal for SDS-PAGE, immunoprecipitation (IP), and protein identification.
Why the 3xFLAG Peptide Matters
In my own research, the shift from single to triple flags was a game-changer. The 3X FLAG peptide provides a much cleaner elution profile. Because of its small size, it does not easily aggregate, and its hydrophilic nature ensures that it remains soluble during the purification process. Whether you are working with 3X FLAG pep The3X FLAG Peptideis a syntheticpeptideof 23 amino acid residue. The Asp-Tyr-Lys-Xaa-Xaa-Asp motif is repeated three times in thepeptide. tide (TFA) formulations or standard salt forms, the consistency of these peptides allows for highly reproducible results in western blotting and downstream protein analysis.
Final Thoughts on Selection
Choosing the right Size for your 3X Flag requirements depends on your throughput. For routine lab work, I usually keep a few vials of 3xFLAG peptide on hand. Always check the certificate of analysis for the molecular weight and ensure the storage Can 3xFLAG peptide (MDYKDHDGDYKDHDIDYKDDDDK) conditions are strictly maintained at -20°C to preserve the integrity of t 3X DYKDDDDK Peptide he 3xFLAG construct. By respecting these technical parameters, you can ensure that your protein purification workflows remain efficient and reliable.