In the realm of biochemical research and molecular studies, the antennapedia peptide sequence has garnered significant attention for its unique structural properties. Often referred to as Penetratin, this specific 16-amino acid sequence is derived from the homeodo TheAntennapediahomeodomain differs at only fiveamino acidpositions from that of Sex combs reduced protein. In a chimeric Antp-Scr proteins expressed Read more main of the *Drosophila* Antennapedia protein. For those interested in the structural nuances of molecular tools, exploring the composition of these chains offers a fascinating look at how spe by W McGinnis·1984·Cited by 1457—Initially identified in cloned copies of the genesAntennapedia, Ultrabithorax and fushi tarazu, thesequenceis also contained within two other Read more cific sequences facilitate interactions at the cellular level.
The Structural Composition of the Sequence
The primary antennapedia peptide is defined by a precise amino acid sequence: Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys. In technical notation, this is often represented as Pept Antennapedia ide sequence: H-RQIKIWFQNRRMKWKK-OH.
When analyzing this peptide, researchers often focus on the specific amino acid arrangement that grants it its functional characteristics. The RQIKIWFQNRRMKWKK chain is a classic example of peptide sequences of various membrane translocating peptides, which have been studied extensively for their ability to interact with biological membranes.
Key technical specifications for this molecule include:
* Molecular Formula: C104H168N34O20S
* Molecular Weight: Approximately 2246.80 Da
* Solubility: Highly soluble in water, making it a versatile tool for experimental setups.
* Modifications: While the native sequence is standard, variants such as {5-FAM}-RQIKIWFQNRRMKWKK (FAM-labeled) are frequently used to track the molecule through fluorescence.
Insights into the Antp Homeodomain
The Antenna-pedia gene was originally discovered in *Drosophila melanogaster*, where it plays a critical role in developmental regulation. The full homeodomain of this protein is a 60-amino acid polypeptide. The 16-mer peptide discussed here corresponds to residues 43–58 of that larger struc Sequence-specific transcription factor which is part of a developmental regulatory system that regulates segmental identity in the mesothorax.Read more ture.
In my experience reviewing literature on sequenced proteins, the precision of the amino acid sequence is paramount. Whether researchers are looking at the native protein or synthesized versions, the sequences must be verified for purity and structural integrity. For instance, studies have shown that Antp is highly expressed in the wing disc in the regions of the notum peptides, highlighting its importance in developmental biology.
Practical Considerations for Research Use
When sourcing these materials, one will often encounter variants such as NH (amide-terminated) versions, which can alter the stability and perform Penetratin ; Molecular Weight, 2246.80 ; Formula, C104H168N34O20S ; Sequence,RQIKIWFQNRRMKWKK; Modifications, None ; Solubility, Soluble in water.Read more ance of the molecule. The Arg-Gln-Ile-Lys-Ile-Trp motif at the N-terminus is particularly noted for its role in the molecule's overall conformational behavior.
Researchers often compare these amino acid sequences against others to Peptide sequence | Chemistry | Research Starters - EBSCO determine binding a A conserved DNA sequence in homoeotic genes of the ffinities or translocation efficiency. Because these peptides are essential for understanding protein-DNA and protein-membrane interactions, maintaining a high standard of chemical purity is essential. Whether you are dealing with the basic 16-mer or a labeled version, the fundamental sequence remains a cornerstone of structural biology.
Summary of Key Data
* Primary Identifier: RQIKIWFQNRRMKWKK
* Category: Cell-penetrating peptide (CPP)
* Origin: *Drosophila* Antennapedia homeodomain
* Research Focus: Membrane interaction and protein transduction mechanisms
By focusing on the rigorous sequencing and structural analysis of the antennapedia peptide, the scientific community continues to uncover the complex ways these molecules function. Whether you are examining the amino acid composition or the broader implications of the Antennapedia gene, each detail provides a deeper understanding of molecular complexity.