In the specialized field of chromatin biology and epigenetic research, the precision of synthetic tools is paramount. As someone who frequently works with protein-based substrates, I Histone H3 Peptide, biotin conjugateThis peptide corresponds to residues 1-21 of histone H3. - Find MSDS or SDS, a COA, data sheets and more information. have found that biotinylated histone H3 peptides serve as indispensable instruments for mapping molecular interactions. These conjugates allow researchers to explore the complex landsca Recombinant human Histone H3encompassing amino acids 2-136 (end). This construct contains an additional cysteine in N-terminal, followed by a His-tag pe of post-translational modifications (PTMs) that regulate gene expression.
The Structural Significance of Histone H3 Peptides
Histone H3 is one of the four core proteins fundamental for compacting eukaryotic DNA into the nucleosome. When we look at a Histone H3 Peptide, biotin conjugate, we are typically dealing wi Histone H3 Peptide, biotin conjugate, This peptide corresponds to residues 1-21 of histone H3. Quality Level. 100. manufacturer/tradename. Upstate®. technique(s). th specific sequences—most commonly residues 1-21 or 21-44—that represent the N-terminal tail of the protein.
In my experience, the choice of peptide length depends entirely on the study objective. For example, a synthesized histone H3 peptide corresponding to residues 1-21 is often used to probe the N-terminal modifications, whereas a synthetic peptide derived from Histone H3.1 covering amino acids 21-44 allows for the investigation of different regulatory domains. These peptides are often purified to >90% via HPLC analysis to ensure high reproducibility in sensitive assays.
Applications in Biochemical Enzymatic Assay Development Histone H3 Peptide Biotinylatedis a high quality peptide corresponding to residues 1-21 of histone H3 and conjugated to biotin. This product has been used as
The primary utility of these molecules lies in their role as a biotinylated histone H3 peptide substrate. Because biotin has an extremely high affinity for streptavidin, these substrates are perfect for high-throughput screening.
* Assay Optimization: Whether you are using a biotinylated histone H3 peptide substrate for a PHF8 (KDM7B) assay or general methyltransferase/deacetylase studies, the biotin tag acts as a versatile anchor.
* Recombinant Alternatives: While synthetic peptides are excellent for site-specific studies, recombinant human histone H3 protein (biotin)—often expressed in *Escherichia coli*—provides a full-length structural context (residues 2-136) that is critical for chromatin a 2 Apr 2012—We present here an in vitro method that combines biotinylation and mass spectrometry (MS) to identify substrates deacetylated by sirtuins. ssembly studies.
Technical Co Immunogen. ThisHistone H3antibody was raised against a syntheticpeptidemade to an C-terminal portion of the humanHistone H3protein (between residues 100- nsiderations and Purity
When sourcing these materials, I always look for specific identifiers, such as Histone H3 (1-21)-GGK(Biotin)-NH2. The inclusion of a GG linker is standard practice, as it provides the necessary spatial flexibility for the biotin moiety to bind to its capture agent without steric hindrance from the histone sequence itself.
It is worth noting that while histone H3 is one of the DNA-b Histone peptides | Life Sciences inding proteins found in the chromatin of all eukaryotic cells, the natural occurrence of biotinylation on these proteins is incredibly low. Studies have indicated that less than 0.001% of human histones H3 and H4 are biotinylated, which makes the use of high-purity synthetic versions essential for any biochemical enzymatic assay. By using a synthetic peptide derived from human histone H3.3 or other variants, we can amplify these signals in a controlled, *in vitro* environment to gain insights that would be impossible to observe at endogenous levels.
Best Practices for Handling
1. Storage: Always follow the manufacturer's COA (Certificate of Analysis) regarding temperature sensitivity.
2. Verification: With products like Histone H3, 1-15 biotin conjugated or the Histone H3 (21-44)-GK(Biotin), ensure that the mass spectrometry data confirms the correct biotinylation site.
3. Experimental Design: When using recombinant histone H3.1 biotinylated (human), keep in mind that the His-tag (often N-terminal) may influence the binding kinetics in certain assay setups.
By focusing on these rigorously characterized tools, researchers can effectively bridge the gap between structural biology and functional genomics. Whether you are working with a short histone H3 (1-18) sequence or a longer fragment, the reliability of your data depends on the quality of the peptide synthesis and the precision of the biotin conjugation.