In the specialized field of molecular biology research, the c-myc 410-419 peptide stands out as a fundamental tool for protein analysis. As someone deeply engaged in laboratory research and the study of synthetic polypeptides, I have frequently utilized this specific sequence to streamline protein detection and purification processes. Understanding the structural and functional nuances of this peptide is essential for any researcher working with epitope tagging.
Understanding the c-myc 410-419 Peptide Structure
The c-myc 410-419 peptide is a synthetic polypeptide derived from the C-terminus of the human c-Myc oncoprotein. Specifically, it encompasses the sequence EQKLISEEDL, which corresponds to amino acids 410-419 of the native protein. This 10-amino acid sequence is highly regarded for its stability and binding affinity.
When analyzing this peptide, it is important to note:
* Sequence composition: N-Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu-C.
* Functional role: It acts as an epitope tag, which is often used as a protein modification to simplify the labeling and detection of target proteins in various assays.
* Research application: It is frequently employed in Western Blotting (WB), Immunoprecipitation (IP), and Immunofluorescence (IF) to validate the expression of recombinant fusion proteins.
The Role of Epitope Tagging in Laboratory Research
The c-Myc-tag is an epitope tag derived from human c-Myc oncoprotein, and its popularity stems The peptide sequence isEQKLISEEDL, corresponding to the C-terminal amino acids (410-419) of human c-myc protein. A complete kit containing this peptide Read more from its small size and high specificity. In my own laboratory experience, I have found that incorporating this tag into recombinant proteins allows for reliable detection using standardized anti-c-Myc antibodies, su Anti-c-Myc epitope (fusion tag) IgG-HRP conjugate ch as the widely recognized 9E10 clone.
Wh Sequence : Epitope sequence, EQKLISEEDL (aa410-419of humanc-mycprotein). Description. The Myc Tag is an epitope tag, commonly used to localize gene products Read more ile PEP-035 is an 11 amino acid synthetic peptide often discussed in commercial catalogs, the core funct THE™ c-Myc Tag Antibody, mAb, Mouse ionality remains rooted in that specific 410-419 region. Whether you are working with c-Myc Peptide (TFA) or lyophilized powder, the goal is to provide a standardized reference for competitive elution or as a positive control in experimental setups.
Practical Considerations for Researchers
When handling these reagents, researchers should be aware of the chemical nature of the c-Myc Peptide. Because it contains many charged residues, solubility is generally favorable in aqueous buffers, though it is always prudent to s The c-Myc-tag synthetic peptidecontains the amino acid sequence which corresponds to the amino acids 410-419of the C-terminal of human c-myc.Read more tart with small-scale dissolution tests in water or recommended assay buffers.
It is fascinating to observe how c-Myc is essential for vasculogenesis and angiogenesis in tumor development; therefore, having precise tools like this synthetic p by A Singh·2022·Cited by 15—Our data indicated effective drug targeting of the disorderedc-Mycprotein via the determination of hot spot residues in thec-Myc/Myc-associated factor X Read more eptide allows us to study the regulatory pathways of such proteins in a controlled *in vitro* environment. The EQKLISEEDL, corresponding to the C-terminal amino acids 410-419 of the human c-myc protein, serves as a critical benchmark.
Final Thoughts on Peptide Selection
For those looking into C myc 410 419 peptide sources, it is vital to ensure the supplier provides high-purity material, often verified via HPLC and mass spectrometry. Whether you are conducting a C myc 410 419 peptide test or integrating it into a broader protein analysis project, the reliability of the sequence is paramount.
The use of c-Myc tag peptide represents a intersection of structural biology and p Human c-Myc peptide (ab166837) ractical biochemistry. By leveraging this specific amino acid residues 410–419 of the human c-Myc protein, we can achieve reproducible results across diverse immunological assays. Always remember that these materials are intended strictly for laboratory research use, providing a robust, non-medical way to track protein behavior and expression patterns in complex cellular systems.