In the realm of biochemical research, the study of peptide fragments has opened diverse pathways for understanding cellular signaling. Among these, CCK tetrapeptide, often referred to as CCK-4 or Tetragastrin, stands out as a critical subject for those investigating gastrointestinal hormones and their synthetic analogs. My personal journey into this field began with an interest in how specific peptide sequences interact at the molecular level, leading me to examine the unique char by K Shiosaki · 1990 · Cited by 50 — cEC50 was determined in the amylase assay as concentration of the peptide that produced 50% of maximal response. potency to both the CCK-A and CCK-B receptors. acteristics of this C-terminal fragment.
Understanding the Structure and Origin
CCK-4 is essentially a carboxyamidated tetrapeptide of CCK, specifically representing the amino acid sequence 30-33 of the larger, 115 amino acid endogenous peptide cholecystokinin. Because it is a synthetic product derived from this complex precursor, it is often utilized Cholecystokinin, CCK Tetrapeptide (30-33). For cellular and molecular biology applications. Available in 1 mg. in cellular and molecular biology applications to isolate specific receptor-binding activities.
Many researchers identify it as Tetragastrin (Cholecystokinin tetrapeptide; CCK-4), which is notable because it is sufficient for binding and eliciting biological activities associated with pancreozymin-like peptides. When I first sourced this material, I noted that it is typically handled as a trifluoroacetate salt, often appearing in a high-purity lyophilized form.
Research Applications and Observations
As a global supplier of CCK-Tetrapeptide (30-33), various institutions provide this compound for strictly non-clinical, analytical research. One of the primary reasons scientists utilize a series of tetrapeptides like CCK-4 is to study the sensitivity of specific receptors.
Interestingly, literature notes that CCK tetrapeptide acts as a potent releaser of insulin and other islet hormones in specific experimental models. My own review of the data suggests that its potency is highly dependent on the experimental environment, often requiring precise solubility in solvents like DMSO.
Behavioral and Neurochemical Context
A fascinating aspect of the literature regarding Cholecystokinin and its smaller fragments involves neurobiology. Some studies have suggested that systemic administration, i by J Bradwejn · 1991 · Cited by 430 — Patients with panic disorder are more sensitive to the panicogenic effect of the peptide than are normal controls. n specific contexts, has increased GABA outflow and induced objective signs of anxiety in laboratory models. Furthermore, it is documented that t by GJ DOCKRAY · 1976 · Cited by 870 — The present study was undertaken to clarify the relationships between the gastrin-like activity in brain and the characteristic forms of gastrin and CCK. his specific peptide can induce panic attacks in humans when administered intravenously during clinical observation, which highlights the profound neurological sensitivity to t Gastrin Tetrapeptide（コレシストキニン テトラペプチド、テトラガストリン、CCK-4）は、ガストリンのC末端テトラペプチドであり、胃液分泌を刺激し、胃粘膜保護作用を持つ his signaling molecule. It is essential to note that these observations are strictly for scientific inquiry and do not imply any application as a supplement or therapeutic agent.
Technical Specifications for Researchers
For those procuring CCK-Tetrapeptide (30-33) for laboratory use, quality control is paramount. The peptide, often indexed under \u82f1\u6587\u540d\u79f0, Cholecystokinin, CCK Tetrapeptide (30-33), should always be accompanied by a certificate of analysis confirming its purity via HPLC.
Key takeaways from my experience include:
* Purity: Ensure the source guarantees >98-99% purity.
* Storage: Always store at -20°C or -80°C to maintain biological integrity.
* Safety: Always treat this as \u7814\u7a76\u7528\u8a66\u85ac\u300cCCK-Tetrapeptide (30-33)\u300d only; it is not intended for any personal use, consumption, or household application.
Summary of Findings
Whether you are exploring CCK signaling pathways or comparing it to the longer CCK-8, the Tetrapeptide remains a cornerstone of gastrointestinal research. As Cholecystokinin is a pe Cholecystokinin: Uses, Interactions, Mechanism of Action | DrugBank ptide hormone of the gastrointestinal system, its truncated forms like CCK-4 provide an elegant way to study the activation of CCK-A and CCK-B receptors without the compl by JF Rehfeld · 2021 · Cited by 39 — The interest in the biological activity of the carboxyamidated tetrapeptide of CCK (Trp-Met-Asp-Phe·CONH2) dates back to the structural identification of exity of the full-length hormone. By maintaining a focus on high-quality, synthetic, and well-vetted reagents, the scientific community continues to uncover the intricate roles these small but powerful molecules play in biological systems.