In the field of biochemical research, the CD47 peptide sequence remains a focal point for those investigating molecular interactions at the cell surface. CD47, often referred to as in CD47, Human tegrin-associated protein (IAP) or MER6, is a transmembrane protein that in humans is encoded by the CD47 gene. As a researcher and enthusiast in the field of synthetic biology, I have spent significant time analyzing the structural nuances of this molecule, which is a ubiquitously expressed membrane protein.
Structural Insights and the CD47 Peptide Sequence
When we look at the molecular architecture, it is important to understand that CD47 is a 40–60 kDa variably glycosylated atypical member of the immunoglobulin superfamily. Its complexity is further highlighted CD47 (CD47) (N-Term) Peptide ; Protein Region. N-Term ; Sequence.FKGRDIYTFD GALNKSTVPT DFSSAKIEVS QLLKGDASLK MDKSDAVSHT; Characteristics. This is a synthetic Read more by its N-linked glycosylation, with critical sites identified at positions 23, 34, 50, 73, 111, and 206.
From a synthetic perspective, researchers often rely on specific fragments to study binding affinities. One of the most common sequences utilized in laboratory settings is:
GNYTCEVTELSREGKTVIELK
This specific custom CD47 peptide containing the sequence of “GNYTCEVTELSREGKTVIELK” is frequently synthesized for use in lipid nanoparticle research and binding assays. It provides a reliable model for exploring how these domains interact with their environment.
Another notable region often explored is the N-terminal fragment. Researchers frequently use a synthetic peptide taken within amino acid region 100-150 on human CD47 protein, characterized by the sequence:
FKGRDIYTFD GALNKSTVPT DFSSAKIEVS QLLKGDASLK MDKSDAVSHT
Understanding the CD47/SIRPα Pathway
The interest in these sequences stems largely from the CD47/SIRPα pathway, an area of intense study in contemporary molecular biology. The CD47 molecule is a transmembrane glycoprotein, which, like MFR, belongs to the superfamily of immunoglobulins. The long isoform precursor of this protein contains 323 amino acid residues, providing a substantial framework for various cellular interactions.
Human CD47-Derived Cyclic Peptides Enhance EngulfmentWhen discussing the primary sequence, it is essential to distinguish between different research applications. For instance, the CD47 Blocking Peptide (A56129) is a standard tool for those looking to perform immunodepletion or immunocompetition experiments. My experience with these materials suggests that purity and the verification of the correct seq by B Li·2023·Cited by 6—CD47/SIRPα pathwayis a new breakthrough in the field of tumor immunity after PD-1/PD-L1. While current monoclonal antibody therapies targeting Read more uence are paramount for consistent results.
Observations on Sequence Variations
The structural-functional diversity of CD47 proteoforms is largely dictated by the precise amino acid arrangement. Whether you are searching for the full sequence in a database or working with a truncated synthetic version, the sequence identity remains a critical variable.
For tho by W Zhang·2024·Cited by 58—The typical self-assembledpeptide, Lys-Leu-Val-Phe-Phe, with asequencederived from Alzheimer's disease-associated β-amyloid, was thepeptideRead more se conducting in-depth analysis, tracking the specific sequence of interest is vital. Whether you are using the “GNYTCEVTELSREGKTVIELK” variant or examining the broader 323 amino acid structure, understanding the 961 - Gene ResultCD47 CD47 molecule [ (human)] underlying biology of CD47 is a fascinating journey. As this field continues to evolve, the ability to synthesize and manipulate these specific peptide segments remains a cornerstone of modern experimental design, allowing for a more granular understanding of how these markers function at the interface of cellular systems.