In the specialized field of biochemical research, the compound frequently referred to as DAPT peptide (CAS Number: 208255-80-5) represents a cornerstone in the study of cellular signaling pathways. As an enthusiast of molecular biology and peptide research, I have spent significant time reviewing the literature surrounding this specific di-peptide derivative to better understand its role in experimental models.
Defining the DAPT Molecule
Scientifically, DAPT (N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester) is widely recognized in laboratory settings as a potent and selective $\gamma$-secretase inhibitor. For those investigating the nuances of protein processing, it is essential to note that DAPT i DAPT is a potent γ-secretase inhibitor. Research studies have demonstrated that DAPT inhibits production of total β-amyloid peptide (Aβ) and Aβ42 in human s a potent $\gamma$-secretase (GS) inhibitor that functions by blocking the production of short amyloid-$\beta$ (A$\beta$) peptides.
When discussing this compound, one must distinguish it from clinical terminology. While the acronym is shared, it is vital to clarify that the research-grade molecule is entirely distinct from the clinical regimen known as "Dual Antiplatelet Therapy," which is a treatment to prevent blood clots that can cause a heart attack or stroke, or a treatment to help stop harmful blood clots from forming. In a research Dual Antiplatelet Medication (DAPT) context, DAPT is a potent and specific inhibitor of $\gamma$-secretase used exclusively in controlled in vitro and in vivo studies.
Mechanisms and Experimental Utility
Th DAPT is an inhibitor of γ-secretase, blocking the production of total Aβ in human primary neuronal cultures with an IC50 value of 115 nM and Aβ42 with an Read more e DAPT | CAS#208255-80-5 | γ-secretase inhibitor primary reason researchers utilize this compound is its ability to modulate specific biological pathways. DAPT blocks Notch signaling which promotes DAPT, gamma-Secretase inhibitor (CAS 208255-80-5) neuronal differentiation of precursor cells. This makes it a highly valuable tool for those studying developmental biology. My personal review of the data suggests that DAPT treatment can influence hematopoietic cell fate decisions, providing a mechanism to observe how cell lineage is determined in embryonic stem cell-derived embryoid bodies.
Key technical parameters include:
* IC50 Values: Research consistently highlights that DAPT is a potent and specific inhibitor of $\gamma$-secretase, with IC50 values of approximately 115 nM for total A$\beta$ and 200 nM for A$\beta$42 in human primary neuronal cultures.
* Molecular Profile: It is a cell-permeable, modified di-peptide with a molecular weight of 432.46 g/mol and a molecular formula of C23H26F2N2O4.
* Pathway Modulation: Because it is an inhibitor of $\gamma$-secretase, it indirectly functions as an inhibitor of Notch, a $\gamma$-secretase substrate.
Innovation in Delivery Sy DAPT blocks Notch signaling which promotes neuronal differentiation of precursor cells4. Enhances iPS cells without oncogenes KLF4 and CMYC5. Cell permeable.Read more stems
In recent years, the integration of this compound into advanced delivery platforms has been a subject of great interest. For instance, studies involving pH sensitive peptide functionalized nanoparticles have demonstrated the potential for co-delivery of therapeutic agents to restrict the progress of cellular proliferation in complex models. This approach highlights how the chemical stability of the DAPT molecule can be preserved and targeted more effectively.
Observations on Research Applications
Beyond its role in Notch signaling, DAPT has neuroprotection activity that continues to be explored in various degenerative disease models. Whether it is being used to investigate the suppression of amyloid-$\beta$ formation or to alter stem cell differentiation, it remains one of the most widely used tools for GSI-IX (gamma-secretase inhibitor IX) research.
While clinical documentation often discusses dual anticoagulant and antiplatelet therapy for coronary artery disease or mentions specific DAPT durations of 6 months after PCI, it is imperative to maintain the distinction that the DAPT peptide discussed here is a biochemical tool. It is a potent and selective $\gamma$-secretase inhibitor designed for laboratory exploration of molecular pathways, providing researchers with the precision required DAPT, gamma-Secretase inhibitor (CAS 208255-80-5) to study complex protein-enzyme interactions without the vari DAPT blocks Notch signaling which promotes neuronal differentiation of precursor cells4. Enhances iPS cells without oncogenes KLF4 and CMYC5. Cell permeable.Read more ables associated with systemic human applications.
By maintaining high standards of purity (typically $\ge$95%) and adhering to established experimental protocols, the study of this peptide continues to yield significant insights into cellular signaling and the fundamental mechanisms of protein processing.