In the sp Dual antiplatelet therapy (DAPT): Managing your symptoms ecialized field of biochemical research, the compound frequently referred to as DAPT peptide (CAS Number: 208255-80-5) represents a cornerstone in the study of cellular signaling pathways. As an enthusiast of molecular biology and peptide research, I have spent significant time reviewing the literature surrounding this specific di-peptide derivative to better understand its role in experimental models.
Defining the DAPT Molecule
Scientifically, DAPT (N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester) is widely recognized in laboratory settings as a potent and selective $\gamma$-secret Comment: DAPTis a γ-secretase inhibitor[1] that is used experimentally to inhibit NOTCH pathway signalling. Chemically it is a modified di-peptide (IUPAC Read more ase inhibitor. For those investigating the nuances of protein processing, it is essential to note that DAPT is a potent $\gamma$-secretase (GS) inhibitor that functions by blocking the production of short amyloid-$\beta$ (A$\beta$) pept DAPT (CAS 208255-80-5)is an inhibitor of γ-secretase. Oral administration of DAPT has been shown to reduce levels of Aβ & to block Notch signaling. ides.
When discussing this compound, one must distinguish it from clinical terminology. While the acronym is shared, it is vital to clarify that the research-grade molecule is entirely distinct from the clinical regimen known as "Dual Antiplatelet Therapy," which is a treatment to prevent blood clots that can cause a heart attack or stroke, or a treatment to help stop harmful blood clots from forming. In a research context, DAPT is a potent and specific inhibitor of $\gamma$-secretase used exclusively in controlled in vitro and in vivo studies.
Mechanisms and Experimental Utility
The primary reason researchers utilize this compound is its ability to modulate specific biological pathways. DAPT blocks Notch signaling which promotes neuronal differentiation of precur Dual Antiplatelet Medication (DAPT) sor cells. This makes it a highly valuable tool for those studying developmental biology. My personal review of th After 1 year, we recommend aDAPTregimen of ASA 81 mg daily plus either ticagrelor 60 mg twice daily or clopidogrel 75 mg once daily (Strong Recommendation, Read more e data suggests that DAPT treatment can influence hematopoietic cell fate decisions, providi DAPT is a potent and specific inhibitor of gamma-secretase. This reduces antibody levels in-vivo in plasma and cerebrospinal fluid.Read more ng a mechanism to observe how cell lineage is determined in embryonic stem cell-derived embryoid bodies.
Key technical parameters include:
* IC50 Values: Research consistently highlights that DAPT is a potent and specific inhibitor of $\gamma$-secretase, with IC50 values of approximately 115 nM for total A$\beta$ and 200 nM for A$\beta$42 in human primary neuronal cultures.
* Molecular Profile: It is a cell-permeable, modified di-peptide with a molecular weight of 432.46 g/mol and a molecular formula of C23H26F2N2O4.
* Pathway Modulation: Because it is an inhibitor of $\gamma$-secretase, it indirectly functions as an inhibitor of N DAPT, also known GSI-IX,is a potent and selective γ-secretase inhibitor. DAPT is a non-transition-state analogue GSI and is the compound most widely Read more otch, a $\gamma$-secretase substrate.
Innovation in Delivery Systems
In recent years, the integration of this compound into advanced delivery platforms has been a subject of great interest. For instance, studies involving pH sensitive peptide functionalized nanoparticles have demonstrated the potential for co-delivery of therapeutic agents to restrict the progress of cellular proliferation in complex models. This approach highlights how the chemical stability of the DAPT molecule can be preserved and targeted more effectively.
Observations on Research by X Wan·2019·Cited by 55—pH sensitive peptide functionalized nanoparticlesfor co-delivery of erlotinib and DAPT to restrict the progress of triple negative breast cancer. Drug Deliv Read more Applications
Beyond its role in Notch signaling, DAPT has neuroprotection activity that continues to be explored in various degenerative disease models. Whether it is being used to investigate the suppression of amyloid-$\beta$ formation or to alter stem cell differentiation, it remains one of the most widely used tools for GSI-IX (gamma-secretase inhibitor IX) research.
While clinical documentation often discusses dual anticoagulant and antiplatelet therapy for coronary artery disease or mentions specific DAPT durations of 6 months after PCI, it is imperative to maintain the distinction that the DAPT peptide discussed here is a biochemical tool. It is a potent and selective $\gamma$-secretase inhibitor designed for laboratory exploration of molecular pathways, providing researchers with the precision required to study complex protein-enzyme interactions without the variables associated with systemic human applications.
By maintaining high standards of purity (typically $\ge$95%) and adhering to established experimental protocols, the study of this peptide continues to yield significant insights into cellular signaling and the fundamental mechanisms of protein processing.