In the world of biochemical research and molecular biology, precision is everything. As someone who has spent significant time working with protein expression vectors and immunological assays, I have frequently turned to the HA peptide kda specifications to ensure the accuracy of my experimental workflows. Understanding the nuances of this small but vital molecule is essential for anyone involved in protein tagging Crystal structure of a peptide complex of anti-influenza peptide antibody Fab 26/9. Comparison of two different antibodies bound to the same peptide antigen.Read more and purification.
What is the HA Peptide?
The HA peptide is a short, nine-amino acid sequence (YPYDVPDYA) derived from an epitope of the human influenza hemagglutinin (HA) protein. This protein is naturally occurring in the virus and is responsible for attaching the virus to cell receptors and initiating infection, a mechanism that allows the virus to target and enter host cells. Because of its high immunoreactivity, it has become a staple in laboratories globally as a reliable tag for fusion proteins.
Key Technical Specifications
When sourc This syntheticpeptideincludingHA-tag sequence has been designed for the elution ofHA-tagged Protein Purification Gel. Thepeptidesequence is YPYDVPDYA.Read more ing this material, the most critical data point for researchers is its size. The HA tag specifically has a molecular weight of 1.1 kDa. This low molec HA-tag peptide [Catalog No.: MBL-3320-205] ular mass is advantageous because it is less likely to interfere with the folding or biological activity of the protein to which it is attached.
From a quality control perspective, I always look for high-purity synthetic versions. Many suppliers offer this peptide with >95% or even >99% HPLC purity. Whether you are The HA-tag is a protein tag derived from the human influenza hemagglutinin (HA) protein, whichallows the virus to target and enter host cells.Read more looking for a ha peptide kda price or evaluating options for sale, always confirm that the product is a high-quality synthetic blocking peptide. Some The Influenza A Hemagglutinin Peptide is a high quality epitope peptide forstimulation of antigen-specific T cellsin T cell assays.Read more versions are explicitly labeled as a synthetic block or HA tag peptide is a synthetic blocking peptide, which is vital when you need to minimize non-specific signals in Western blotting or other immunoassays.
Practical Applications in t The secreted recombinant Influenza A virus (A/California/04/2009 (H1N1))HA(mutated) comprises 527 amino acids and predicts a molecular mass of 60kDa.Read more he Lab
My experience with this molecule primarily centers on its role in competitive binding. The HA peptide is a highly immunoreactive tag that is commonly used to elute HA fusion protein bound to Anti-HA antibody during immunoprecipitation. By providing a high concentration of the free peptide, you can facilitate the release of your target protein from the antibody-bead complex under neutral pH conditions, which is much gentler than low-pH elution methods.
Furthermore, it is used for competing out the anti-HA antibody binding in various immunoassays. This is particularly useful in verifying the specificity of an antibody-antigen interaction. By pre-incubating the antibody with the peptide, you can effectively block the binding site, confirming that the observed signal is indeed specific to the HA tag.
Why Precision Matters
Beyond simple elution, the Influenza Hemagglutinin (HA) Peptide is a versatile tool. I have seen it utilized in structural studies, such as the crystal structure of a peptide complex of anti-influenza peptide antibody Fab 26/9, which provides deep insight into how these antibodies recognize their epitopes. Additionally, in immunological research, variants of this peptide are employed for the stimulation of antigen-specific T cells in specialized assays.
Final Thoughts on Handlin Native Isolation of 3×HA‐Tagged Protein Complexes to g
When working with this reagent, remember that it is derived from the human influenza hemagglutinin protein, making it a robust and well-characterized tool. Whether you are dealing with a kDa measurement in the context of a 1.1 kDa tag or a larger 60 kDa fusion protein, the reliability of the HA tag remains unmatched. Always store your aliquots according to the manufacturer’s instructions—usually in PBS or a similar buffer—to maintain the integrity of the peptide sequence.
By focusing on the HA peptide as a standardized tool, researchers can ensure consistent results across different expression systems, from simple cell lysates to complex protein purifications. It remains one of the most efficient and cost-effective ways to manage fusion protein detection and isolation in modern molecular biology.