In the world of biochemical research and molecular biology, precision is everything. As someone who has spent significant time working with protein expres ChromoTek HA-Peptide ; Conjugate, Unconjugated ;Molecular Weight, 1.1 kDa; Purity, 95% ; Reconstitution, Reconstitute peptide in 1550 uL PBS, which results in a Read more sion vectors and immunological assays, I have frequently turned to the HA peptide kda specifications to ensure the HA Peptide is a highly immunoreactive taggenerally used for the separation of tagged proteins from cell culture supernatants and cell lysate under neutral pH Read more accuracy of my experimental workflows. Understanding the nuances of this small but vital molecule is essential for anyone involved in protein tagging and purification.
What is the HA Peptide?
The HA peptide is a short, nine-amino acid sequence (YPYDVPDYA) derived from an epitope Influenza Hemagglutinin (HA) Peptide HA tag peptide of the human influenza hemagglutinin (HA) protein. This protein is naturally occurring in the virus and is responsible for attaching the virus to cell receptors and initiating infection, a mechanism that allows the virus to target and enter host c HA Peptide; CAS 92000-76-5 ells. Because of its high immunoreactivity, it has become a staple in laboratories globally as a reliab Recombinant H1N1 Hemagglutinin/HA Protein, 11055- le tag for fusion proteins.
Key Technical Specifications
When sourcing this material, the most critical data point for researchers is its size. The HA tag specifically has a molecular weight of 1.1 kDa. This low molecular mass is advantageous because it is less likely to interfere with the folding or biological activity of the protein to which it is attached.
From a quality control perspective, I always look for high-purity synthetic versions. Many suppliers offer this peptide with >95% or even >99% HPLC purity. Whether you are looking for a ha peptide kda price or evaluating options for sale, always confirm that the product is a high-quality synthetic blocking peptide. Some versions are explicitly labeled as a synthetic block or HA tag peptide is a synthetic blocking peptide, which is vital when you need to minimize non-specific signals in Western blotting or other immunoassays.
Practical Applications in the Lab
My experience with this molecule primarily centers on its role in competitive binding. The HA peptide is a highly immunoreactive tag that is commonly used to elute HA fusion protein bound to Anti-HA antibody during immunoprecipitation. By providing a high concentration of the free peptide, you can facilitate the release of your target protein from the antibody-bead complex under neutral pH conditions, which is much gentler than low-pH elution methods.
Furthermore, it is used for competing out the anti-HA antibody binding in various immunoassays. This is particularly useful in verifying the specificity of an antibody-antigen interaction. By pre-incubating the antibody with the peptide, you can effectively block the binding site, confirming that the observed signal is indeed specific to the HA tag.
Why Precision Matters
Beyond simple elution, the Influenza Hemagglutinin (HA) Peptide is a versatile tool. I have seen it utilized in structural studies, such as the crystal structure of a peptide complex of anti-influenza peptide antibody Fab 26/9, which provides deep insight into how these antibodies recognize their epitopes. Additionally, in immunological research, variants of this peptide are employed for the stimulation of antigen-specific T cells in specialized assays.
Final Thoughts on Handling
When working with this reagent, remember that it is derived from the human influenza hemagglutinin protein, making it a robust and well-characterized tool. Whether you are dealing with a kDa measurement in the context of a 1.1 kDa tag or a larger 60 kDa fusion protein, the Antigen, Hemeagglutinin-tag (HA-tag) represents a 9-aapeptidesequence (designated HA11-P; controlpeptide) from hemagglutinin influenza virus (aa 114-122; Read more reliability of the HA tag remains unmatched. Always store your aliquots according to the manufacturer’s instructions—usually in PBS or a similar buffer—to maintain the integrity of the peptide sequence.
By focusing on the HA peptide as a standardized tool, researchers can ensure consistent results across dif ChromoTek HA-Peptide ferent expression systems, from simple cell lysates to complex protein purifications. It remains one of the most efficient and cost-effective ways to manage fusion protein detection and isolation in modern molecular biology.