In the realm of biochemica HA tag peptide (ab13835). Secondary. All lanes: Alexa Fluor 680 Goat-anti-Rabbit IgG at 1/5000 dilution.Predicted band size: 64 kDa. Observed band size: 55 kDa.Read more l research, the precision of molecular tools is paramount. When working with epitope tagging, the HA peptide stands out as a foundational component for researchers requiring reliable protein detection and purification. Understanding the technical nuances of this molecule, particularly its size and structural properties, is essential for e HA peptide xperimental design.
Defining the HA Peptide
The HA peptide is a synthetic sequence derived from the hemagglutinin protein of the influenza virus. It is widely utilized in laboratory settings due to its high specificity for the anti-HA monoclonal antibody (clone 12CA5). Its primary utility lies in its ability to act as an epitope tag, allowing for the identification or isolation of fusion proteins without significantly altering their native conformation or biological activity.
Technical Breakdown: Molecular Weight and Size
When sourcing these materials, researchers often encounter varying data points regarding its HA Peptide mass. The standard molecular weight is consistently cited in the range of 1102.15 to 1102.18 g/mol. This consistency reflects the HA peptide for Elution high-purity synthetic nature of the peptide, which is typically composed of the nine-amino-acid sequence: Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala.
From a structural perspective, the HA-tag is small, which is a significant advantage in molecular biology. Because the tag is only nine amino acids long, it minimizes the risk of steric hindrance or interference with the folding of the protein of interest. Often, the predicted band size of a tagged protein (such as 64 kDa) remains largely unaffected by the addition of the tag, provided the fusion is designed correctly.
Practical Research Applications
Researchers frequently source this material in multiple pack sizes to accommodate different experimental scales. Common offerings include:
* 1 mg vials for small-scale pilot studies.
* 5 mg quantities, often provided as a formulation: 5 mg purified peptide (1mg/mL), which is ideal for standard elution procedures.
* 20.0 mg bulk options for large-scale affinity chromatography or purification runs.
For those calculating precise molarity, it is helpful to note that specific documentation often lists concentrations such as 1 mM, 0.9073 mL for dilution series. Whether you are performing competitive elution of fusion proteins from immobilized beads or using the peptide for Western blotting controls, having a clear understanding of the 1102.18 or 1102.15 mass ensure HA tag peptide C-YPYDVPDYA [LT8282] - 0.00 s that stoichiometric calculations remain accurate.
Quality and Storage Considerations
The peptide is typically supplied as a lyophilized powder, which offers excellent stability when stored at -20°C. When reconstituting, it is common practice to use PBS (Phosphate Buffered Saline) to achieve a working concentration, such as 1 mg/mL. Because the molecular weight of 1102.15 is well-defined, researchers can easily determine the required volume for their specific size requirements.
Summary of Specificati Like other epitope tags,HA-tag is smalland generally does not alter the traits of proteins it is attached to. As a result HA-tags are often used to identify Read more ons
* Common Molecular Weight: ~1102.18 g/mol
* Sequence: YPYDVPDYA
* Primary Use: Epitope tagging and competitive elution
* Physical State: Lyophilized powder (typically >95% purity)
By maintaining a focus on these technical benchmarks—specifically the 1,102.18 g/mol mass and the compact nature of the HA sequence—investigators can ensure that their experimental results are both reproducible and robust. While the 1102.1 g Formulation: 5 mg purified Peptide (1mg/mL) no preservative; 26184 detects HA in samples. 26184 has been successfully used in Control procedures.Read more /mol figure is standard, always verify the specific certificate of analysis provided by the supplier to ensure your experimental size needs are met with the necessary precision.
*Note: All products mentioned are for laboratory research use only. This content is for informational purposes for researchers and does not constitute medical advice or recommendations for human use.*