In the field of cellular analysis, the selection of specific reagents is paramount for achieving high-fidelity results. As a researcher frequently involved in characterizing cell surface markers, I have spent significant time evaluating the HIB19 clone antigenic peptide and its corresponding monoclonal antibody. Understanding the nuance between different clones is essential for those of us working with B-lymphocyte populations.
Understanding th CD19 Antibody (HIB19) [Alexa Fluor® 488] e HIB19 Entity
The HIB19 monoclonal antibody is a foundational tool in laboratory settings. It is widely recognized as an IgG1 κ mouse monoclonal CD19 antibody that specifically targets the human CD19 protein. Scientifically, CD19 is considered the earliest and broadest B cell-restricted antigen, making it an indispensable marker for identifying B-cell lineages throughout their development.
The protein itself is a 95 kDa transmembrane glycoprotein. My experience with this clone consistently shows that it reacts with human CD19 with high specificity. When discussing the technical specifications, it is important to note that this clone is raised against B-lymphocyte antigen CD19, ensuring that it serves as a robust phenotypic marker in flow cytometry and immunohistochemistry.
Technical Performance and Comparative Analysis
One of the most frequently asked questions in our community is how Anti-Human CD19 Antibody, Clone HIB19 HIB19 compares to other options like SJ25-C1 or 4G7. It is well-documented that clones SJ25-C1 and HIB19 recognize overlapping epitopes. Because of this, Clone HIB19 partially blocks anti-human CD19 clones such as 4G7 and SJ25C1.
In my own experimental setups, I have observed that Anti-CD19 clone HIB19 is exceptionally reliable for surface staining. Whether using the STEM A monoclonal mouse antibody, supplied by Invitrogen Antibodies,raised against B-lymphocyte antigen CD19(Human), cited in 61 publications, with 1 published Read more CELL Technologies Anti-Human CD19 Antibody, Clone HIB19 or other commercial variations like the Mouse anti-Human CD19 Monoclonal Antibody (Clone HIB19), the consistency of the binding remains a hallmark of this reagent.
Why CD19 Matters in Research
The biological significance of this antigen cannot be overstated. CD19 lowers the threshold for antigen receptor-dependent stimulation, which allows B cells to respond precisely to their environment. Because it is widely used as a p TotalSeq™-D0050 anti-human CD19 Antibody henotypic marker for CD19 Buy anti-CD19 antibody,Mouse anti-Human CD19 Monoclonal Antibody (Clone HIB19) (MBS696919) product datasheet at MyBioSource, Primary Antibodies. expression on B cells, it remains a staple in any lab analyzing peripheral blood lymphocytes or developmental stages of B cells.
Regarding the HIB19 clone antigenic peptide and its application, users should be aware that the Purified Anti-Human CD19 Antibody[HIB19] is often preferred for specialized assays where secondary detection or custom conjugation is required. These commonly used mouse monoclonal antibodies against human CD19 have paved the way for standardized research protocols globally.
Personal Observations
When selecting reagents, I prioritize those with a proven track record. The HIB19 clone has demonstrated, through years of peer-reviewed literature, that it is a robust choice. If you are performing flow cytometry, ensure you are aware that this reagent reacts with CD19 consistently, but always verify your specific fluorochrome conjugation (such as PE, APC, or Alexa Fl CD19 Monoclonal Antibody (HIB19), eBioscience™ uor) to avoid spectral overlap with other markers in your panel.
For those inquiring about Hib19 clone antigenic peptide positive results or seeking information on Anti CD19 monoclonal antibody and Anti mouse CD19 variants, always refer to the specific datasheet provided Mouse anti-Human CD19 Monoclonal Antibody by the manufacturer. The precision of these tools is what allows us to push the boundaries of molecular research without ambiguity.