In the specialized field of molecular biology, the study of circadian regulation has become increasingly sophisticated. Researchers frequently investigate the KAT13D / CLOCK peptide as a vital tool CLOCK clock circadian regulator KAT13D bHLHe8 for structural analysis and antibody validation. By focusing on the molecular underpinnings of the circadian rhythm, scientists can Anti-KAT13D / CLOCK antibody (ab3517) better understand how these proteins govern metabolism and cellular timing.
The Nature of the KAT13D / CLOCK Peptide
The CLOCK gene, also known as KAT13D or *Circadian Locomotor Output Cycles Kaput*, encodes a basic helix-loop-helix (bHLH)-PAS transcription factor. This protein plays a central role in the regulation of circadian rhythms. In research settings, a synthetic peptide derived from this sequence—often corresponding to specific regions such as the N-terminus or amino acids 450–500—is utilized as a critical reagent.
Whether one is working with a Rabbit polyclonal antibody to KAT13D/CLOCK or a Goat Polyclonal KAT13D/CLOCK antibody, the use of a Clock Blocking Peptide is essential for ensuring experimental specificity. These peptides may be used for neutralization and control experiments, allowing researchers to confirm that the observed signal in techniques like Western Blot or Immunohistochemistry is specific to the target protein.
Technical Specifications and Applications
When selecting a synthetic peptide within Human CLOCK for your laboratory requirements, it is important to consider the immunogen design. Many high-quality reagent Function. ARNTL/2-CLOCKheterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadianclock.Read more s are developed using a synthesi Clock Circadian Regulatorinformation. Aliases. Clock Circadian Regulator is also known as AAC53200, bHLHe8, CLOCK, hCLOCK, KAT13D, KIAA0334, mCLOCK, rCLOCK.Read more zed peptide derived from human CLOCK, w 5 citations – A rabbit monoclonal antibody, raised against Circadian locomoter output cycles protein kaput (Human), supplied by Abcam. hich is then used to generate polyclonal or monoclonal antibodies.
Key technical observations include:
* Molecular Weight: The native protein typically exhibits an observed molecular weight of approximately 95 kDa.
* Cross-Reactivity: Many Rabbit polyclonal KAT13D / CLOCK antibody products are validated for use across multiple species, including human, mouse, and rat samples.
* Experimental Utility: Beyond standard blotting, these reagents are instrumental in studies regarding time-dependent protein expression.
In my experience with laboratory workflows, the inclusion of a Mouse KAT13D / CLOCK Antibody or similar variants is standard practice when analyzing the Clock Circadian Regulator across different model systems. Because the protein regulates circadian rhythm and metabolism, maintaining high-grade controls is non-negotiable.
Emerging Research Trends
The utility of these tools extends into cutting-edge by JE Lee·2013·Cited by 81—Here we explore variations in the expression levels of neuropeptides present in the SCN of rats using a label-free quantification approach that Read more oncology and chronobiology. Recent studies have highlighted how specific compounds can target circadian clock proteins hijacked by glioblastoma stem cells, effectively impairing the survival and growth of those cells. Furthermore, research into \u751f\u7269\u949fKAT13D\u86cb\u767d\u6297\u4f53 (biochemical clock protein antibodies) has provided deeper insights into how the peptide sequence interacts with other transcriptional machinery.
Whether you are performing a synthetic peptide, corresponding to a region within amino acids 450 mapping study or simply seeking to validate a new batch of antibodies, the precision of the peptide sequence is paramount. The rigorous application of these tools ensures that the complex data derived from clock protein interaction studies remain reproducible and scientifically sound, ultimately contributing to our broader understanding of the biological systems that dictate our daily cycles.