In the realm of biochemical research an C-Peptide, Insulin, Proinsulin and Interpretations d synthetic peptide analysis, understanding the struc C-Peptide | Peptide Synthetic | High Purity tural properties of specific chains is essential. One frequently analyzed component is the connecting peptide, commonly referred to as the molecular weight c peptide. For researchers and enthusiasts focusing on protein synthesis and structural biology, identifying the precise mass and C-Peptide formula of this 31-amino-acid polypeptide is a foundational step in ensuring the integrity of experimental models.
Defining the Entity: What is C-Peptide?
The C-peptide is a short, 31-amino-acid polypeptide that serves as an essential structural bridge, connecting the A-chain and B-chain within the proinsulin molecule. From a biochemical perspective, this molecule is vital for the correct folding of proinsulin. While historically considered biologically inert, contemporary research highlights its role in cellular signaling and regulatory functions.
When sourcing this material for laboratory applications, researchers often encounter the specific molecular formula C129H211N35O48. The standard molecular weight for human C-peptide is widely cited in literature as approximately 3020.3 g/mol.
Analyzing Technical Specifications and Variations
When reviewing technical documentation or Certificates of Analysis (CoA), you may notice slight variations in reported figures depending on the specific synthesis or purification method used. Here is a breakdown of the 13 Jan 2026—Molecular Formula: C129H211N35O48 ;Molecular Weight: 3020.3; MDL Number: MFCD00076295 ; MOL File: 33017-11-7.mol ; MSDS File: SDS.Read more common data points observed in current research:
* Standard Molecular Weight: Most high-purity synthetic batches report a mass of 3020.3 g/mol.
* Alternative Measurements: You might occasionally see values such as 3020.26, 3020.27, or 3020.5 depending on the precision of the mass spectrometry used by the manufacturer.
* Dalton Conversions: It is frequently referred to as having a mass of approximately 3021 daltons or simply C-Peptide (Human) | 33017-11-7 | PCP-3725-PI 3020 Dalton.
* Proinsulin Variants: It is important to distinguish between the standard 31-amino-acid chain and larger precursor fragments, such as Proinsulin C-Peptide (55-89), which carries a significantly higher MW of 3616.99.
For those working in cellular and molecular biology, maintaining strict adherence to these technical parameters is crucial. When preparing stock solutions, always reference the batch-specific data provided by the supplier. For instance, while water solubility is common for many peptides, some C-peptide variants may require specific solvents like dimethyl sulfoxide (DMSO) for optimal stability.
Practical Considerations for Researchers
In my personal experience with peptide proc C-PEPTIDE (HUMAN) | 33017-11-7 urement, verifying the MW is the first step in quality control. Whether you are investigating the molecular weight c peptide for structural studies or as a reference standard, sourcing from reputable suppliers who provide clear documentation on MOLECULAR FORMULA and purity (typically >95% via HPLC) is non-negotiable.
Key Takeaways for Your Research:
1. Consistency: The C peptide is a well-defined peptide structure. If your source material deviates sign Biochemistry, C Peptide - StatPearls - NCBI Bookshelf - NIH ificantly from the 3020.3 g/mol benchmark, it may indicate a different fragment or an impure batch.
2. Storage: To maintain the integrity of this molecular structure, standard storage conditions of -20 ± 5 °C are generally recommended.
3. Application: Always ensure your experimental design accounts for the specific molecular weight listed on the vial label, as this is the only verifiable metric for calculating precise molar concentrations.
By focusing on these technical details—understanding the molecular properties and the specific C peptide sequence—researchers can effectively navigate the complexities of peptide synthesis and structural analysis. Whether you are dealing with the standard 3020.3 g/mol human variant or studying the broader molecular context of proinsulin, precision in measurement remains the cornerstone of reliable results.