In the realm of molecular biology and protein characterization, the myc peptide sequence remains one of the most significant tools for researchers. As someone who has spent considerable time exploring synthetic peptides for laboratory-based protein expression and purification studies, I have found that understanding the intricacies of this sequence is essential for anyone working with recombinant DNA technology.
Defining the Myc Peptide Sequence
The standard myc peptide sequence is derived from the c-Myc protein, specifically corresponding to the amino acids 410-419 of the C-terminal of human c-Myc. This short, ten-amino-acid motif, represented by the sequence EQKLISEEDL, is widely utilized as an epitope tag.
When analyzing the amino acid sequence, we see it follows the structure: Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu. This specific arrangement of residues provides the necessary immunoreactivity that makes the Myc tag a small, immunoreactive peptide tag highly valued in Western blots and co-immunoprecipitation experiments.
Technical Specifications
* Sequence: EQKLISEEDL
* Residue Range: 410-419 (C-terminal)
* Molecular Weight: Approximately 1,203.31 Da
* Human c-Myc peptide characteristics: Often synthesized as a blocking peptide or for antibody validation.
Practical Considerations for Laboratory Use
For those working in the lab, it is important to note that the Myc peptide is often supplied as a lyophilized powder. When preparing this for sequencing or binding assays, I have found that proper storage is critical. Because peptides containing cysteine—often added to the N-terminus to facilitate conjugation, such as (C)-E410-Q-K-L-I-S-E-E-D-L419—are easily oxidized, they should always be prepared in solution immediately prior to use to ensure stability.
Interestingly, the Myc tag monoclonal antibody 9E10 is the gold standard for recognizing this epitope, though its binding can be influenced by the ne 24 Apr 2020—The Myc and c-Myc tag are two terms for the same tag, which is derived from the c-Myc protein andhas a peptide sequence of EQKLISEEDL.Read more ighboring structure. This is why when using vectors like pCMV-Myc-N, one must be mindful of the surrounding protein environment to ensure the tag remains accessible.
The Role of the Myc Protein in Biology
Be Of Myc and Men yond its use as a tag, the c-Myc protein itself is a fascinating transcription factor. Research indicates that it specific Formula. C53H87F3N12O ; CAS No. 2918768-02-0 ; Appearance. Solid ; Color. White to off-white ; Sequence.Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu.Read more ally recognizes the core DNA sequence 5'-CAC[GA]TG-3'. It is a nuclear phosphoprotein that plays a fundamental role in cell cycle progression and cellular transformation.
Furthermore, the Myc protein contains a nuclear localization sequence (NLS) comprising residues Pro320 to Asp328, which is essential for its translocation into the nucleus. My experienc Formula. C53H87F3N12O ; CAS No. 2918768-02-0 ; Appearance. Solid ; Color. White to off-white ; Sequence.Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu.Read more e with these sequences Human Myc Tag (aa 410-419) Synthetic Peptide (PEP-035) has highlighted how even small segments, like the 410-419 epitope, can be leveraged for high-precision protein characterization without interfering with the biological function of the parent protein.
Summary of Findings
Whether you are performing C-Myc peptide epitope VAC-00374 testing or simply trying to understand the 410-419 sequence of the C-terminal of human c-Myc protein, the reliability of the EQKLISEEDL motif is unmatched. It remains a staple in the field, offering a robust method to track and purify proteins in complex cellular environments.
By paying attention to the specific amino acid composition Myc-tag: An epitope tag for protein characterization, a c-Myc Peptide nd the chemical properties—such as the potential for pyroglutamyl formation at the N-terminus when Glutamine or Glutamic acid is present—researchers can optimize their protocols for better results. The Myc tag continues to be a versatile and indispensable component for high-quality protein research.