In the realm of molecular biology and protein characterization, the myc peptide sequence remains one of the most significant tools for researchers. As someone who has spent considerable time exploring synthetic peptides for laboratory-based protein expression and purification studies, I have found that understanding the intricacies of this sequence is essential for anyone working with recombinant DNA technology.
Defining the Myc Peptide c-Myc Peptide (M2435) Sequence
The standard myc peptide sequence is derived from the c-Myc protein, specifically corresponding to the amino acids 410-419 of the C-terminal of human c-My MYC Gene - GeneCards | MYC Protein | MYC Antibody c. This short, ten-amino-acid motif, represented by the sequence EQKLISEEDL, is widely utilized as an epitope tag.
When analyzing the amino acid sequence, we see it follows the structure: Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu. This specific arrangement of residues provides the necessary immunoreactivity that makes the Myc tag a small, immunoreactive peptide tag highly valued in Western blots and co-immunoprecipitation experiments.
Technical Specifications
* Sequence: EQKLISEEDL
* Residue Range: 410-419 (C-terminal)
* Molecular Weight: Approximately 1,203.31 Da
* Human c-Myc peptide characteristics: Often synthesized as a blocking peptide or for antibody validation.
Practical Considerations for Laboratory Use
For those working in the lab, it is important to note that the Myc peptide is often supplied as a lyophilized powder. When preparing this for sequencing or binding assays, I have found that proper storage is critical. Because peptides containing cysteine—often added to the N-terminus to facilitate conjugation, such as (C)-E410-Q-K-L-I-S-E-E-D-L419—are easily oxidized, they should always be prepared in solution immediately prior to use to ensure stability.
Interestingly, the Myc tag monoclonal antibody 9E10 is the gold standard for recognizing this epitope, though its binding can be influenced by the neighboring structure. This is why when using vectors like pCMV-Myc-N, one must be mindful of the surrounding protein environment to ensure the tag remains accessible.
The Role of the Myc Protein in Biology
Beyond its use as a tag, the c-Myc protein itself is a fascinating transcription factor. Research indicates that it specifically recognizes the core DNA sequenc Human c-Myc peptide (ab166837) e 5'-CAC[GA]TG-3'. It is a nuclear phosphoprotein that plays a fundamental role in cell cycle progression and cellular transformation.
Furthermore, the Myc protein contains a nuclear localization sequence (NLS) comprising residues Pro320 to Asp328, which is essential for its translocation into the nucleus. My experience with these sequences has highlighted how even c-Myc tag peptide is a synthetic peptide with a molecular weight of 1,203 Da. The peptide sequence isEQKLISEEDL, corresponding to the C-terminal amino acids.Read more small segments, like the 410-419 epitope, can be leveraged for high-precision protein characterization without interfering with the biological function of the parent protein.
Summary of Findings
Whether you are performing C-Myc peptide epitope VAC-00374 testing or simply trying to understand the 410-419 sequence of t c-Myc Peptide (M2435) he C-terminal of human c-Myc protein, the reliability of the EQKLISEEDL motif is unmatched. It remains a staple in the field, by B Rizzuti·2022·Cited by 6—Myc has a nuclear localization sequence (NLS) comprising residues Pro320 to Asp328, to allow for nuclear translocation. We designed a peptide comprising such Read more offering a robust method to track and purify proteins in complex cellular environments.
By paying attention to the specific amino acid composition and the chemical properties—such as the potential for pyroglutamyl formation at the N-terminus pCMV-Myc Sequence and Map when Glutamine or Glutamic acid is present—researchers can optimize their protocols for better results. The Myc tag continues to be a versatile and indispensable component for high-quality protein research.