In the realm of molecular biology and protein characterization, the myc tag peptide sequence serves as a cornerstone for researchers aiming to isolate, detect, and analyze recombinant proteins. As someone who frequently w What is the use of myc tag in plasmids with interest gene? orks with synthetic peptides for laboratory research, I have found that understanding the structural nuances of the Myc tag is essential for successful experimental design.
Defining the Myc Tag Structure
The Myc tag is a small, immunoreactive peptide tag derived from the c-terminal of the c-myc gene. The standard sequence, which is widely recognized in professional settings, is EQKLISEEDL. This 10-amino acid sequence is equivalent to the three-letter code: Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu.
When working with this specific sequence, it is important to note its physical characteristics. The c-Myc Peptide is a synthetic peptide with a molecular weight of 1203.3 Da. Its precise composition, often represented as H-Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu-OH, makes it a highly effective tool for protein labeling and discovery.
Why the Sequence Matters
The efficacy of this tag is largely dependent on the Myc tag monoclonal antibody 9E10, which was historically generated against this specific epitope. However, experience shows that the Myc-tag-binder is the sequence LISEEDL. Within this core motif, the amino acid residues isoleucine (I), aspartic acid (D), and the last leucine (L) are essential for maintaining a strong binding affinity.
Researchers often encounter the term "c-Myc tag" and "Myc tag" interchangeably; they refer to the same entity, which is an epitope tag coded by the gene fragment corresponding to the C-terminal amino acids Glu-410 to Leu-419 of the human c-Myc protein.
Technical Specifications for Researchers
For those utilizing vectors such as pcDNA3.1+ C-Myc, precision is key. Whether you are using a standard 10-amino acid peptide or a variant like PEP-035 (an 11-amino acid synthetic peptide), the sequence corresponds to the same functional region.
* Molecular Weight: Approximately 1203.3 Da. Myc proto-oncogene protein - Homo sapiens (Human)
* Sequence: EQKLISEEDL.
* Origin: Derived from human c-Myc protein (amino acids 410-419).
* Recognition: Effectively detected The calmodulin-bindingpeptide(CBP) is a short 26-residuesequencederived from the C-terminus of skeletal muscle myosin light chain kinase that binds Read more by specialized antibodies in Western blots and co-immunoprecipitation assays.
Practical Considerations
When incorporating the Myc tag into your research, consider the following:
1. Context Matters: The Myc tag monoclonal antibody 9E10 displays highly variable MYC-Targeting Inhibitors Generated from a Stereodiversified epitope recognition depending on the neighboring sequence context. If your tagged protein is misfolded or the tag is buried, detection sensitivity may drop.
2. Synthetic Purity: Always verify the purity of your c-Myc Peptide (TFA) or other synthetic variants. High-purity white powder forms are standard for ensuring reproducible results in buffer-based assays.
3. DNA Binding: While the tag itself is a protein modification tool, the parent protein (c-Myc) is a transcription factor that specifically recognizes the c Myc-tag ore sequence 5'-CAC[GA]TG-3'. Keeping this in mind helps distinguish the tag's function from the protein's native biological activity.
The Myc tag remains a gold standard because it is a short amino acid sequence (EQKLISEEDL) tha His tag peptide - Cosmo Bio USA t rarely interferes with the function or folding of the target protein. By foc The c-Myc Peptideis a syntheticpeptidewith an amino acidsequencethat corresponds to the amino acids 410-419 of the C-terminal of human c-myc.Read more using on the Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu motif, you ensure that your experimental protocols remain consistent with the broader scientific literature. Whether you are performing purification or simple protein tracking, this peptide remains one of the most reliable tools available.