In the specialized field of immunology and cellular biology research, the NLVPMVATV peptide occupies a significant position. As someone who has spent considerable time exploring laboratory reagents and their specific applications, I have found that understanding the precise nature of these molecular tools is essential for any rigorous investigative work.
Defining the NLVPMVATV Peptide
The NLVPMVATV peptide is a well-characterized, linear peptidic epitope derived from the 65 kDa phosphoprotein (pp65) of the human cytomegalovirus (HCMV). Specifically, this sequence corresponds to the amino acid range 495-503. In the context of molecu Data Sheet CMV pp65 peptide NLVPMVATV lar biology, it is widely recognized as a dominant epitope presented by the HLA-A*02:01 allele, which is a key component of the major histocompatibility complex (MHC) Class I system.
When sourcing this material, researchers often encounter the HCMV pp65 (495-503) peptide in various forms, ranging from HCMV pp65 495-503 (HLA-A*02:01) NLVPMVATV lyophilized powder to complexed reagents. The molecular mass of this specific antige PresentER-NLVPMVATV (GFP) (Plasmid #102947) n is approximately 943.2 Da, a detail that is critical when calculating molar concentrations for *in vitro* stimulation assays.
Practical Applications and Research Insights
My experience with high-purity reagents has shown that the quality of the peptide is paramount to achieving reproducible data. Whether utilizing synthetic native peptide (NLVPMVATV) for surface loading on antigen-presenting cells or employing Peptide-loaded MHC MACSimers, the precision of the sequence synthesis determines the reliability of the output.
For those focusing on T-cell receptor (TCR) interaction studies, the NLVPMVATV peptide acts as a standardized tool. Researchers often use this single peptide (NLVPMVATV) to stimulate CD8+ T-cells to observe specific immune recognition patterns. It is fascinating to note that this specific sequence is thought to be the dominant peptide that is presented in all HLA–A*0201 individuals, making it a "gold standard" for immune monitoring and a Human cytomegalovirus (CMV) is one of the major causes of severe complications in immunocompromised patients, such as recipients of hematopoietic cells, ssay development.
Advanced Experimental Tools
In the laboratory, we frequently look for more sophisticated delivery or detection methods. For instance, t by E Wagner-Drouet · 2021 · Cited by 8 — Cytomegalovirus (CMV) infection is a major cause of morbidity and mortality following hematopoietic stem cell transplantation (HSCT). he Plasmid PresentER-NLVPMVATV (GFP) has become a valuable asset for those looking to express the epitope within a cellular environment. Additionally, when looking for Antigen Peptide CMV pp65 - HLA-A*0201 (NLVPMVATV), one must ensure the product meets strict purity standards—typically >97% as verified by HPLC—to avoid non-specific background interference in cellular assays.
For those working with larger experimental designs, there are various peptides available in pools, but for targeted studies, the isolated Peptidfragment (NLVPMVATV) remains the most MHC MACSimer A*0201 CMV pp65 (NLVPMVATV), human reliable for isolating the specific response of HLA-A*0201-restricted T-cells.
Final Thoughts on Quality and Sourcing
When evaluating different suppliers, I prioritize companies that provide comprehensive documentation, such as Certificates of Analysis (CoA) and detailed mass spectrometry data. Given that this is a highly specific tool, the consistency of the NLVPMVATV sequence is non-negotiable. Whether you are conducting epitope mapping or assessing the binding affinity of novel TCR-like antibodies, utilizing a high-grade NLVPMVATV peptide ensures that your experimental variables remain controlled.
By focusing on these specific, verifiable molecular entities, researchers can continue to advance our collective under Antigen Peptide CMV pp65 - HLA-A*0201 (NLVPMVATV) · Currency: · GBP · EUR. £556.00. add to basket. Shipping is calculated in checkout. Product details. Purity standing of immune recognition mechanisms without relying on anecdotal or unverified materials. Always ensure that the reagents used are validated for their intended research purpose to maintain the integrity of your laboratory investigations.