In the realm of immunological research, the OVA peptide SIINFEKL—specifically the sequence corresponding to residues 257-264 of the chicken ovalbumin protein—stands as a cornerstone for laboratory investigations. As someone who frequently works with these reagents, I have found that understanding the nuances of this specific epitope is essential for anyone involved in high-precision cellular assays.
Defining the OVA Peptide (257-264)
The OVA peptide (257-264), commonly identified by its amino acid sequence SIINFEKL, is an immunodominant epitope derived from the ovalbumin protein found in egg whites. With a molecular weight of approximately 963.13 g/mol and the chemical formula OVA Peptide (257-264) C45H74N10O13, this peptide is highly valued for its stability and specificity. My experience with high-purity OVA 257-264 (SIINFEKL) has shown that it is the gold standard for researchers aiming to achieve consistent results in murine models.
Applications in Research
The primary utility of this molecule lies in its ability to be presented by the MHC class I molecule, H-2Kb. Because of this, the ovalbumin-derived peptide SIINFEKL is routinely used to stimulate ovalbumin-specific T cells. When I conduct experiments, I rely on this peptide to serve as a reliable positive control. It is used to stimulate T cells in PBMCs and plays a critical role in quantifying peptide epitope specificity, often measured through IFN-γ releasing effector cells using an ELISPOT assay.
For those looking into methods to monitor antigen presentation, this peptide is indispensable. It allows for the tracking of H-2Kb complexes, particularly when utilizing specialized tools like the 25-D1.16 monoclonal antibody, which selectively reacts with the ovalbumin-derived peptide with sequence SIINFEKL when bound to the MHC class I molecule.
Variations and Structural Considerations
While the native SIINFEKL sequence is the standard, researchers often explore variants to test structural specificity. One common alternative is the OVA G4 peptide, which is a variant of the agonist ovalbumin (OVA) peptide SIINFEKL The 25-D1.16 monoclonal antibody reacts with mouse MHC class I H-2Kb bound to theovalbumin-derived peptide with sequence SIINFEKL.Read more . The G4 peptide (SIIGFEKL) is a variant of the agonist ovalbumin (OVA) peptide that helps in mapping the structural requirements for T-cell activation.
Whether you are working with the standard single peptide (SIINFEKL) for stimulation of murine Ovalbumin-specific CD8+ T-cells or investigating modified versions, the purity of the material is paramount. I have found that sourcing high-quality, synthetic versions—often provided as a TFA salt—ensures that the SIINFEKL peptide behaves predictably in vitro.
Practical Insights and Observa The 25-D1.16 monoclonal antibody reacts with theovalbumin-derived peptide SIINFEKLbound to H-2Kb of MHC class I, but not with unbound H-2Kb.Read more tions
When setting up experiments, it is vital to remember that Ovalbumin (OVA) is the primary protein in egg-white and is inherently immunogenic. Consequently, the SIINFEKL peptide is widely utilized in fundamental studies of antigen processing. From my own observations, when cells are exogenously pulsed with the SIINFEKL peptide, the detection of MHC I-peptide complexes becomes significantly more robust, especiall Ova peptide – siinfekl s7951 y when cells are pretreated with cytokines like IFN-γ.
If you are just beginning to work with OVA peptide SIINFEKL, ensure that you ar 2 days ago—At the heart of this antibody's function is its recognition of the ovalbumin-derived peptideSIINFEKLH-2Kb OVA peptide SIINFEKL· PE Anti-Mouse Read more e aware of the specific storage requirements for your OVA peptide (257-264) to maintain its structural integrity. Whether you are performing a basic stimulation assay or m by I Rastogi·2025·Cited by 6—Immunization with native or β-modifiedSIINFEKL peptidesdid not result in tumor suppression or improved survival. C57BL/6 mice (n = 5 per group, 25 mice total) Read more ore complex kinetic studies, the interaction between this peptide and the H-2Kb molecule remains one of the most reliable systems in modern immunology.
By utilizing these well-characterized sequences, researchers can ensure that their SIINFEKL peptides provide the necessary consistency required for reproducible results in all OVA peptide (257-264)-relat OVA (257-264) H-2Kb Peptide (SIINFEKL) | p&e ed research.