In the world of biochem RGD Peptide ical research and biomaterial development, the RGD peptide stands out as a fundamental tool. As someone who frequently works with synthetic peptides for cell adhesion studies, I have learned that understanding the precise molecular weight of your specific compound is not just a technicality—it is essential for accurate experimental design.
Decoding the RGD Peptide MW
When searching for the rgd peptide mw, you will quickly notice Molecular Weight. 593.62 g/mol. Short Term Storage. store at <-15°C, keep under inert gas: Nitrogen. Storage. store at <-15°C, keep under inert gas: Nitrogen. that the values are not universal. Thi Find rgd peptide and related products for scientific research at MilliporeSigma. s is becaus Chemical Information, Storage & Stability. Molecular Weight, 614.61, Formula. C23H38N10O10. e "RGD" refers to a specific three-amino-acid sequence—Arginylglycylaspartic acid—but researchers often use various derivatives, cyclic structures, or extended sequences to achieve specific binding affinities.
The most standard, linear tripeptide (Arg-Gly-Asp) typically has a molecular weight of 346.34 Da (or 346.344 g·mol⁻¹). If you see a product labeled with this 346.34 value, you are likely looking at the basic, unmodified form. However, as my own experience in the lab has shown, modifications change everything.
For instance, variants like GRGDNP often used in integrin inhibition have a much higher MW: 614.6 (or 614.61 g/mol). Meanwhile, other specialized forms, such as those used for specific surface functionalization, might present a molecular weight of 593.62 g/mol. Even cyclic RGD structures, which are highly stable, often show a mass around 612.7 g/mol.
Why Molecular Weight Matters in Practice
When I am preparing my stock solutions, I rely heavily on the molecular weight to calculate the exact concentration. Whether you are using a peptide calculator or manual math, the formula remains consistent: Mass (in grams) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol).
Because RGD peptides are often supplied as lyophilized powders, checking the specific M.Wt, 614.6 or 346.34 Da listed on the Certificate of Analysis provided by the manufacturer is the only way to ensure your molarity is correct.
Applications and Integrin Signaling
The reason we obsess over these numbers is the function of the peptide itself. RGD peptides are used to study cell adhesion mechanisms and integrin signalling pathways. These molecules mimic the cell attachment sequences found in extracellular matrix proteins like fibronectin and vitronectin.
In my work, I have found that synthetic integrin-binding inhibitors are incredibly effective at modulating cell-matrix interactions. Furthermore, in more advanced research, RGD peptides can be utilized to specifically target cancer cells by engaging with integrin receptors Chemical Properties of RGD Peptide ; M.Wt, 614.6 ; Solubility, DMF: 14 mg/ml,DMSO: 5 mg/ml,PBS (pH 7.2): 0.33 mg/ml ; General tips on the cell surface.
Selecting the Right Product
If you are looking to source high-quality materials, keep these factors in mind:
* Purity: Always look for high purity (typically >95% or >97%).
* Solubility: Note that solubility varies significantly. For example, some forms are highly soluble in water (≥17.3 mg/mL), while others require DMSO or PBS.
* Storage: Always store your peptides according to the manufacturer’s instructions—often at <-15°C under inert gas like Nitrogen to prevent degradation.
Products like PEPTITE-2000 is a synthetic peptide containing the RGD cell attachment sequence, which I have used in the past, provide excellent consistency. Whether you are conducting basic research on cell migration PEPTITE-2000 is a synthetic peptide containing the RGD cell attachment sequence found in fibronectin, vitronectin and many other matrix and serum proteins. or complex tissue engineering, knowing if your rgd peptide is the standard 346.34 or a The RGD motif is a cell adhesion sequence that binds to integrins, a receptor family for extracellular matrix proteins. We previously reported that the larger derivative is the first step toward reproducible results.
By paying attention to the specific molecular weight and the structural modifications of your RGD peptides, you ensure that your experiments remain robust, accurate, and scientifically sound.