In the specialized field of immunology research, the SLLMWITQC peptide holds a significant position as a well-characteriz Antigen Peptide NY-ESO-1 - HLA-A*0201 (SLLMWITQC) for antigen-specific stimulation in T cell assays such as ELISPOT, ICS or proliferation assays, ed epitope. Derived from the New York esophageal squamous cell carcinoma 1 (NY-ESO-1) protein—a prominent cancer/testis antigen found in *Homo sapiens*—this nonapeptide has become a standard tool for scientists studying cellular interactions and MHC-restricted responses.
Understanding the SLLMWITQC Peptide Structure
The SLLMWITQC peptide (157-165) is a linear peptidic epitope that functions as an essential component in biochemical assays. My experience with this specific sequence highlights its utility in studying HLA-A*02:01 restricted interactions. Because it is a WT NY-ESO-1/HLA*02:01-restricted peptide, SLLMWITQC, it serves as a reliable model for researchers examining how specific antigens are presented by the MHC Class I complex.
When investigating this molecule, one must consider its structural integrity. Crystal structure analysis of the NY-ESO-1 epitope, SLLMWITQC, has provided de by X Liu·2022·Cited by 55—NY- ESO- 1157- 165 (SLLMWITQC) monomer. The same numbers of. A2- ESO TCR T- and 2D2 -CAR- T cells were cocultured with CMV pp65 495- 503peptideor NY- ESO- 1 Read more tailed insights into how it binds within the HLA-A2 groove, a critical step for molecular modeling and assay validation.
Research Applications and Assay Utility
The Antigen Peptide NY-ESO-1 - HLA-A*0201 (SLLMWITQC) is frequently utilized in high-precision laboratory settings. I have observed that its primary role involves:
* Antigen-specific stimulation: It is instrumental in T-cell assays, inclu Development of a TCR- like antibody and chimeric antigen ding ELISPOT and intracellular cytokine staining (ICS).
* MHC Complex Studies: The Biotinylated Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) complex protein is a sophisticated reagent often used in flow cytometry to identify antigen-specific populations.
* Sensitivity Tools: For those requiring maximum detection sensitivity, peptide-loaded MHC MACSimers offer a robust platform for reliable data Peptide-loaded MHC MACSimersprovide maximum detection sensitivity and reliability for reproducible identification and analysis of antigen-specific T cells via Read more collection.
Whether you are using a standard peptide or a specialized construct such as the SLLMWITQC + AIB(C9) variant, consistency is key. The 157-165 SLLMWITQC region is highly conserved, making it a focal point for those studying the NY-ESO-1/LAGE-1-derived SLLMWITQC peptide interactions in variou Multiepitope CD8+ T cell response to a NY-ESO-1 peptide s models.
Comparative Studies and Analogs
In my review of available materials, I’ve noted that researchers often compare the wild-type sequence with analogs. For instance, the NY-ESO-1 (157-165) C165V SLLMWITQV is a common alternative. While SLLMWITQC is the benchmark, the single peptide (SLLMWITQV) is also widely used for the stimulation of human CD8+ T-cells where specific binding affinities are being tested.
Furthermore, the NY-ESO-1 SPEAR T cells produced cytokines in response to antigen-specific stimulation using the SLLMWITQC sequence, demonstrating the biological relevance of this epitope in controlled laboratory environments. This hig PE-Labeled Human HLA-A*02:01&B2M&NY-ESO-1 hlights why it is classified as a class, class I, animal species, human reagent—its applicability across various humanized test systems is extensive.
Concluding Thoughts o NY-ESO-1 (157-165) peptide - SLLMWITQC n Technical Integration
Whether you are utilizing a recombinant human single-chain TCR (scTCR) 1G4 to detect the epitope or conducting topical peptide immunization with NY-ESO-1B peptide (SLLMWITQC), the precision of the peptide source is paramount. As SLLMWITQC, an NY-ESO-1-derived peptide, continues to be a subject of intense study, it remains a cornerstone for understanding the fundamental mechanisms of the tumor cell antigen found in various types of cancers.
For anyone working with the HLA-A*02:01 negative tetramer-APC or similar conjugates, the SLLMWITQC from Homo sapiens (human) NY-ESO-1 protein remains the gold standard for verifying experimental accuracy in non-clinical research. Always ensure that your inventory of NY-ESO-1 peptide (SLLMWITQC) is sourced from reputable providers to maintain the integrity of your peptide-based investigations.