When exploring the world of biochemical research and synthetic p Tatstands for "Trans-Activator of Transcription". The protein consists of between 86 and 101 amino acids depending on the subtype.Tatvastly increases the Read more eptides, precision is paramount. As someone who frequently works with specialized reagents, I have found that understanding the nuances of a TAT peptide is essential for anyone involved in cell-culture studies. One of the most common questions I encounter pertains to the tat peptide molecular weight, as this value can fluctuate significantly depending on the specific sequence length and chemical modifications involved.
Defining the TAT Peptide
The TAT peptide, derived from the trans-activator of transcription protein of HIV-1, is a well-known cell-penetrating peptide (CPP). It is widely utilized in laboratory settings for its remarkable ability to facilitate the entry of various molecules into cells. Because the protein itself consists of 86 to 101 a Tat (HIV) mino acids, researchers typically synthesize shorter, functional segments—most notably the 47-57 or 49-57 regions—to achieve efficient membrane translocation.
Analyzing Variations in Molecular Weight
In my experience, you cannot rely on a single number when discussing this compound. The molecular weight is entirely dependent on the specific amino acid sequence and w HIV-1 Tat Protein Peptideis a cell-penetrating peptide used to mediate gene delivery to cells in culture. CAS Number: 191936-91-1. Molecular Weight:.Read more hether the peptide is amidated or conjugated. Here is a breakdown of the data points I often reference:
* Standard HIV-1 Tat Protein Peptide (47-57): This is the most common version. You will frequently see a molecular weight of 1559.85 g/mol or 1559.83 g/mol, with a molecular formula of C64H118N32O14.
* Variations and Conjugates: If you are using a FAM-labeled peptide, the MW (average) can spike to 2031.27. Conversely, shorter segments like the 49-57 sequence may have a weight of 1339.63.
* Full-Length Considerations: While the functional segments are small, the full TAT protein is much larger, with a molecular weight of 14 kDa.
* Specific Batch Data: I always advise checking the Certificate of Analysis (CoA) for your specific lot. Some synthetic versions may show a weight of 1621.91 or even 1662.97 depending on purity and terminal modifications.
Why Precision Matters
Whether you are performing a tat peptide molecule study or calculating stock solu Tat (HIV) tions, using the exact mass is non-negotiable. I often use a Tat peptide molecular weight calculator to ensure my molarity is correct. For example, when working with a molecular weight of 1559.8 g/mol, a slight deviation in your mass measurement can lead to significant errors in your final concentration.
For those curious about the physical properties, you will typically receive this substance as a white to off-white lyophilized powder. It is generally soluble in water, but I always verify the storage conditions—usually -20°C—to maintain the stability of the 1559.9 gram/mole structure.
Practical Tips for Researchers
When you are sourcing these materials, you might come across terms like 1,432.76 g/mol or even higher weights like 3472.97 for complex TAT-D1 variants. Always cross-reference the molecular formula provided by your supplier. If you are comparing a molecular mass 1560 Da product against a 2350.8 g/mol (WT) variant, ensure your experimental design accounts for these differences. TAT Peptide ; Purity, ≥99% ; Molecular Weight,1621.91; Molecular Formula, C65H124N34O15 ; Appearance, Solid ; Solubility, Soluble in water.Read more
In my personal workflow, I have found that maintaining a log of the 1621.90 or 1621.91 values—which are common for standard analytical-grade TAT—helps me keep my buffers consistent. Whether you are using HIV-1 Tat Peptide for custom conjugat TAT peptide (WT) | C95H172N42O24S2 - PubChem - NIH e synthesis or basic uptake assays, the key is to treat the molecular weight as a variable that must be verified on every vial.
By paying close attention to these technical specifications—from the formula C64H118N32O14 to the specific 1339.63 weight of shorter fragments—you ensure that your laboratory results are both reproducible and scientificall TAT peptide y sound.