In the specialized field of biochemical research, the TAT peptide remains one of the most widely studied tools for cellular interaction studies. As someone who has spent significant time Tat (HIV) sourc TAT: All About Cell Penetrating Peptides ing and analyzing various peptides for experimental workflows, I have found that understanding the specific molecular weight (mw) of an oligopeptide or a protein is essential for accurate titration and successful outcomes.
Clarifying the TAT Peptide MW Variations
When you look for a TAT peptide, you will quickly notice that the molecular weight (mw) is not a single, static figure. This is because TAT peptides are a subset of cell-penetrating peptides derived from different regions of the original HIV-1 protein. Depending on the sequence length and modifications, the mass can vary significantly:
* TAT (47-57): This shorter, truncated version is a frequent choice for researchers. You will often see its MW : 1 559.89 Da or approximately 1558.88 g/mol cited in documentation.
* Full-length or modified sequences: Other variants, such as those labeled with specific purity standards, often list an MW of 1621.91 or even 1662.97.
* Complex Conjugates: When dealing with specialized forms like HIV-1 Tat Protein Peptide, you might encounter higher mass values, such as 2350.8 g/mol, or smaller synthesized fragments like 1,432.76 g/mol for clickable variants.
It is important to note that the full HIV-1 Tat protein itself is a by A Subrizi · 2012 · Cited by 55 — Our study aimed to explore the relationship between structure and activity of Tat peptide and its uptake mechanism. For this purpose we transcriptional activator that increases RNA Pol II processivity and has a much higher molecular weight of 14 kDa. When researchers refer to the TAT peptide as a cell penetrating peptide, they are almost exclusively working with the basic domain (typically residues 47-57 or 49-57) rather than the full protein.
Practical Considerations for Researchers
My experience with Tat has taught me that precision in ordering is vital. Whether you are searching for TAT peptide mw data or evaluating a Tat peptide sequence for a custom synthesis, always verify the sequence against the provided mass. The sequence GRKKRRQRRRPQ is the most common motif associated with these delivery systems.
Why Mass Matters The cysteinyl moiety at position 1 of the C-TAT peptide contributes largely to the destabilizing potency and the penetration efficacy of impermeant substances. in Experimental Design
The molecular weight dictates the molar concentration during your experiments. Because Tat acts as a regulatory protein that facilitates the translocation of c by Z Maani · 2024 · Cited by 12 — TAT, a multifunctional viral protein with a molecular weight of 14 kDa, serves as an RNA-binding protein. It enhances the expression of viral genes by argoes across cellular membranes, consistency in the mass used is the difference between a successful uptake study and inconsistent data.
I have observed that even minor variations in the synthesis process—such as the inclusion of a terminal cysteine for conjugation—will shift the 1,559.89 Da baseline. Always check the certificate of analysis provided by the supplier to ensure your calculations align with the specific batch you receive.
Summary of HIV-1 Tat Protein Peptide (CAS 191936-91-1) Findings
While the HIV-1 Tat peptide is a powerful tool, it is not a one-size-fits-all reagent. From the standard 1621.91 weight to the specific 1559.83 fragments, researchers must treat each as a distinct entity. As a cell penetrating peptide, its ability to move cargo is highly dependent on the integrity of the basic arginine-rich sequence.
When you are ready to source these materials, remember that the Tat nomenclature often refers to the HIV transactivator domain. Always confirm the specific sequence and the corresponding molecular weight before proceeding with your protocols. This attention to detail ensures that the peptides function as intended, maintaining the high standards required in biochemical research.