In the world of biochemical research and peptide synthesis, few motifs have garnered as much interest as the YIGSR peptide sequence by N Yoshida·1999·Cited by 39—TheYIGSR (Tyr–Ile–Gly–Ser–Arg) laminin β1 chain sequencehas an inhibitory effect on tumour growth and the metastasis of melanoma and . As someone who actively follows advancements in extracellular matrix (ECM) mimetics, I have found the study of this specific pentapeptide to be incredibly fascinating. Whether you are exploring its role in cell-matrix interactions or its potential in advanced material science, understanding the fundamental structure of this molecule is essential.
Decoding the YIGSR Sequence
At its core, the YIGSR peptide is a short, functional motif derived from the basement membrane protein laminin. Specifically, it represents the 929–933 sequence of the β1 chain. The sequence one The pentapeptide Tyr-Ile-Gly-Ser-Arg (YIGSR) occurs from amino acids 929-933 in the protein laminin. The.YIGSR sequence is the major receptor binding siteRead more letter code is simply Y-I-G- YIGSR (Laminin Fragment 929-933) | Polypeptide S-R, which translates to the amino acid chain: Tyr-Ile-Gly-Ser-Arg.
To break it down further, this pentapeptide consists of Tyrosine, Isoleucine, Glycine, Serine, and Arginine. When looking at the molecular weight, it typically sits around 594.67 g/mol for the standard form. Researchers often utilize the Laminin Peptide YIGSR-NH2 (amidated ve Peptides: Emerging Candidates for the Prevention and Treatment of rsion), which has a slightly different molecular weight of approximately 593.67 Da, often preferred for its enhanced stability in specific experimental c DSPE-PEG1000-YIGSRis a PEG compound which composed of DSPE and a biomimetic peptide YIGSR. YIGSR interacts with the 67 kDa laminin binding protein (LBP) onditions.
The Role of Laminin Fragment 929-933
The YIGSR (Laminin Fragment 929-933) is widely recognized as a major receptor-binding site. In my experience reviewing literature on this topic, it is clear that this sequence found on the B1 chain of laminin acts as a crucial signal for cell adhesion and migration.
Many researchers focus on the YIGSR sequence is the major receptor binding site because of how it interacts with the 67 kDa laminin-binding protein. This interaction is what makes it such a pow by M Lakshmanan·2007·Cited by 4—The nativesequence YIGSR-myr and the mutatedpeptideYIGSD-myr show nearly the same area while the others show large changes in the area, potential and Read more erful tool in studies involving cell-adhesive activity. Whether it is used in its native form or integrated into complex delivery systems like DSPE-PEG1000-YIGSR, the specificity of this Tyr-Ile-Gly-Ser-Arg motif remains its most significant attribute.
Comparative Insights: YIGSR vs. Other Sequences
When comparing various bioactive peptides, you will often see YIGSR mentioned alongside others like IKVAV (from the α1 chain). While both are vital for understanding how cells organize into tissues, the YIGSR within the B1 chain is uniquely studied for its specific inhibitory effects on cell by Z Meng·2018·Cited by 37—The peptideTAT-H6-K(C18)-YIGSRdisplayed transfection efficiencies at N/P ratios of 6, which was 3.5 and 7 times higher than that of Lipo 2000 in B16F10 and Read more -matrix intera The laminin-derived peptide YIGSR (Tyr-Ile-Gly-Ser-Arg ctions.
It is interesting to note how variations in the sequence YIGSR can alter performance. For instance, in some advanced gene transfection studies, researchers have utilized the TAT-H6-K(C18)-YIGSR construct to improve efficiency. This demonstrates that the YIGSR (Tyr–Ile–Gly–Ser–Arg) laminin β1 chain sequence is not just a passive structural component, but a versatile functional unit.
Practical Considerations for Handling
If you are sourcing this for research, you will frequently encounter the pentapeptide in a lyophilized powder form. Most high-quality suppliers provide the sequence, YIGSR with a purity level of ≥98%.
When storing these materials, standard protocols usually recommend:
* Temperature: -20°C for long-term stability.
* Solubility: The peptide is generally soluble in water, though specific buffers may be required depending on your experimental design.
* Verification: Always cross-reference the CAS registry number (110590-64-2) to ensure you are working with the correct Tyr-Ile-Gly-Ser-Arg isomer.
Final Reflections
The YIGSR sequence continues to be a cornerstone in the study of structural biology. By focusing on the 929–933 sequence of the β1 chain, scientists can better understand the complex signaling pathways involved in tissue development and cell behavior. As more sequences are discovered, the YIGSR motif remains a reliable standard for those of us interested in the intersection of synthetic chemistry and biological function.
Whether you are looking at the pentapeptide YIGSR for its adhesive properties or its role in signaling, the precision of this Tyr-Ile-Gly-Ser-Arg chain highlights the incredible specificity found within the laminin protein structure. Always ensure you are sourcing from reputable providers to maintain the integrity of your peptide research.